They said it related to an alleged failure by the company to comply with laws that require Airbnb to pay Italian tax authorities 21% of landlords' rental income in the country.
The requirement dates back to an EU Court of Justice ruling in 2017 that was unsuccessfully appealed by Airbnb earlier this year.
The money seizure order, which covers the years 2017-2021, targets Airbnb's Ireland unit and also three individuals who held managerial roles at the company during that timeframe.
Airbnb has yet to comment.
The company had fought the tax crackdown, which demanded Airbnb and rivals to provide the authorities details of all their rental contracts in Italy, on the grounds it broke EU principles on the freedom to operate across the bloc.
